Genome‐wide analysis of DNA copy number alterations in early and advanced gastric cancers

摘要: To better understand progressive changes in gastric cancer (GC), early and advanced GCs (EGC AGC, respectively) were examined for copy number alterations (CNAs). A crypt isolation method was used to isolate DNA from tumors normal glands 20 AGCs, fresh tumor samples obtained 45 EGCs. We assessed CNAs differentiated-type using an Infinium HumanCytoSNP-12v2.1 BeadChip EGCs AGCs. The most frequent aberrations EGC gains at 8q23.3 (42.2%) 8q23.2 (40%), loss of heterozygosity (LOH) 3p14.2 (24.2%), suggesting that these involved the development EGC. On other hand, highest frequencies AGC found 8q24.21 (65%) 8q24.3 (60%). LOHs 11q24.3-25, 11q23.2-24.1, 11q14.1, 12p11.21-13.33, whereas 3p14.2. In addition, regions copy-neutral detected 11q21, 11q13.3-14.3, 11q11, 11p13-15.3, 12q21.1, 12q12-13.3 5q33.3-35.1. Comparisons showed significant differences 12q22-q23.2, 12q21.33, 11p12, 11p14.1, 12q21.31-32.32, 3p12.3, 3p14.1, 10p15.1, 1q24.2 2q12.1. Copy neutral significantly higher than 14q32.11-32.33, 14q21.3, 14q11.2, 5q11.2, 5q 13.3, 14q21.1-23.2, 14q13.2-13.3, 5q12.1-12.3, 5q11.1, 17p13.3. total lengths greater pattern quite different suggest increasing numbers are associated with disease progression AGC. © 2016 Wiley Periodicals, Inc.