Inhibition of mesodermal fate by Xenopus HNF3β/FoxA2

摘要: Abstract The winged-helix transcription factor HNF3β/FoxA2 is expressed in embryonic organizing centers of the gastrulating mouse, frog, fish, and chick. In HNF3β required for formation mammalian node notochord, can induce ectopic floor plate when misexpressed developing neural tube; expression extraembryonic endoderm also necessary proper morphogenesis primitive streak. frog Xenopus laevis , several lines evidence suggest that related Pintallavis functions as ortholog both axial mesoderm neurectoderm; role itself, however, has not been clearly defined, subject this study. widely vegetal pole but, previously suggested, excluded from gastrula-stage mesoderm. We find an HNF3β-Engrailed repressor fusion protein induces axes inhibits head embryos, while anterior explant assays vivo. Our studies target genes function to limit extent gastrula, point roles component during vertebrate gastrulation.