Сравнительное исследование поведения клеток спинального ганглия крысы и линии РС12 на поверхностях, модифицированных биоадгезивными полимерами

摘要: We studied the adsorption of bioadhesive polymers (polyornithine, gelatin, laminin) on polystyrene surface by use dynamic light scattering. The contribution biopolymers to resulting zeta potential modified was assessed. PC12 cells do not exhibit selective adhesion in presence foetal bovine serum. Polystyrene with adsorbed polyornithine promotes primary cultured serum-free medium nerve growth factor. Subsequently laminin induces spreading and differentiation into neuronal direction. Primary neurons isolated from rat spinal ganglion adhere preferentially polyornithine-modified surface. On polyornithine-laminin intensively form neuritis that correlates proliferation glial positive for S100 protein. results show similar response material latter being more sensitive this Isolated cell culture can be used study relationship between neurite outgrowth Schwann different biomaterials.