Aligned electrospun nanofibers specify the direction of dorsal root ganglia neurite growth.

摘要: Nerve injury, a significant cause of disability, may be treated more effectively using nerve guidance channels containing longitudinally aligned fibers. Aligned, electrospun nanofibers direct the neurite growth immor- talized neural stem cells, demonstrating potential for directing regenerating neurites. However, no study neu- rite on these fibers has yet been performed with primary neurons. Here, we examined neurites from dorsal root ganglia explants poly-L-lactate nano- high, intermediate, and random alignment. On fibers, grew radially outward turned to follow upon contact. Neurite was robust, never leaving grow surrounding cover slip. To com- pare alignment that nanofiber substrates, Fourier methods were used quantify alignment, however striking, infe- rior fiber all but randomly Neurites highly substrates 20 16% longer than intermediate respectively. Schwann cells assumed very narrow morphology compared those sur- rounding coverslip. The robust demon- strated here is step toward use aligned, regeneration. 2007 Wiley Periodicals, Inc. J Biomed Mater Res 83A: 636-645,