Raman spectroscopy reveals differences in collagen secondary structure which relate to the levels of mineralisation in bones that have evolved for different functions
作者： Kevin Buckley , Pavel Matousek , Anthony W. Parker , Allen E. Goodship
DOI: 10.1002/JRS.4038
关键词:
摘要: Bone is a composite material comprising collagen fibril scaffold surrounded by crystals of carbonated-hydroxyapatite mineral. It well established that the relative proportions mineral and in mature bone are not definite adapted order to ‘tune’ its mechanical properties. known, however, how ratio controlled. This paper uses Raman spectroscopy (which permits probing both phases bone) explore hypothesis control mechanism related nature bones with different levels mineralisation have qualitatively collagen. spectra functionally varying presented features indicate differences collagen's secondary structure (amide I band profiles) post-translational modification (hydroxyproline/proline ratios) highlighted. The study demonstrates can provide means investigate mechanisms bone. Understanding these could pave way towards therapeutic alteration and, thus, properties Copyright © 2012 John Wiley & Sons, Ltd.
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