Doses effects of zoledronic acid on mineral apatite and collagen quality of newly-formed bone in the rat's calvaria defect.

摘要: Due to their inhibitory effects on resorption, bisphosphonates are widely used in the treatment of diseases associated an extensive bone loss. Yet, little is known about newly-formed quality. In present study, adult male Sprague-Dawley rats (n=80) with a defect calvaria area were and short-term zoledronic acid (ZA) studied healing area. Three ZA treatments tested by using either: 1°) low single dose (120μgZA/kg, n=10; equivalent human osteoporosis treatment), 2°) fractionated doses (20μgZA/kg daily for 6days either total 120μg/kg, n=15), 3°) high doses, (100μgZA/kg weekly 6weeks, n=15; 6months metastasis treatment). For each treatment, control "vehicle" was performed (with identical number rats). After administration, intrinsic material properties evaluated quantitative backscattered electron imaging (qBEI) Raman microspectroscopy. Neither nor modify compared respective animals. On opposite, resulted significant decrease crystallinity (-25%, P< 0.05) hydroxyproline-to-proline ratio (-30%, P<0.05) bones. Moreover, positively correlated (ρ=0.78, P<0.0001). The data highlight new formation craniofacial model. As such, at disrupted apatite crystal organization. addition, we report here first time that decreased suggesting may affect early collagen organization during healing.