Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis

摘要: The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due the opacity of adult fish. To address this, we have created transparent in which transplanted either hematopoietic stem/progenitor cells or tumor cells. In hematopoiesis radiation recovery assay, transplantation GFP-labeled marrow allowed striking vivo visual assessment engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both wild-type fish had equal engraftment, this could only be visualized recipient. model, RAS-melanoma visualization proliferation, distant metastases as little 5 days, not seen recipients until 3 4 weeks. This serves ideal combination sensitivity resolution cell analyses.