EFFECTS OF IRRADIATION ON BRAIN VASCULATURE USING AN IN SITU TUMOR MODEL

摘要: Purpose Damage to normal tissue is a limiting factor in clinical radiotherapy (RT). We tested the hypothesis that presence of tumor alters response tissues irradiation using rat situ brain model. Methods and Materials Intravital microscopy was used with cranial window assess effect C6 glioma on peritumoral without RT. The RT regimen included 40 Gy at 8 Gy/day starting Day 5 after implant. Endpoints blood–brain barrier permeability, clearance index, leukocyte-endothelial interactions staining for vascular endothelial growth (VEGF) glial fibrillary acidic protein, apoptosis. To characterize system RT, animal survival surface area volume were measured. Sham experiments performed similar animals implanted basement membrane matrix absent cells. Results alone increases permeability but has little leukocyte–endothelial astrogliosis. Radiation interactions, highest levels cell adhesion seen model combined irradiation; however, appeared reduce rolling leukocytes. Unirradiated had poor clearance. Irradiated index irradiated unirradiated sham-implanted animals. reduces VEGF did not affect amount apoptosis tissue. Apoptosis identified radiation. Conclusions developed novel approach demonstrate intracranial irradiation.