Respirometry as a tool to quantify kinetic parameters of microalgal mixotrophic growth

摘要: Modeling microalgal mixotrophy is challenging, as the regulation of algal metabolism affected by many environmental factors. A reliable tool to simulate behavior in complex systems, such wastewaters, may help setting proper values operative variables, provided that model parameters have been properly evaluated. In this work, a new respirometric protocol proposed quickly obtain half-saturation constant for several nutrients. The was first verified autotrophic exploitation ammonium and phosphorus (Monod kinetics), well light intensity (Haldane model), further elaborated on specific supply basis. It then applied measure kinetic heterotrophic growth. constants nitrogen resulted comparable with ones. dependence acetate dissolved oxygen concentration assessed. Mixotrophy modeled combination autotrophic/heterotrophic reactions, implemented AQUASIM, validated batch curves with/without bubbling, under nutrient limitation, different intensities. shown reliability useful nutrients, therefore perform bioprocess simulation.