Bidirectional global spontaneous network activity precedes the canonical unidirectional circuit organization in the developing hippocampus

摘要: Spontaneous network activity is believed to sculpt developing neural circuits. giant depolarizing potentials (GDPs) were first identified with single-cell recordings from rat CA3 pyramidal neurons, but here we identify and characterize a large-scale spontaneous term global activation (GNA) in the mouse hippocampal slices, which measured macroscopically by fast voltage-sensitive dye imaging. The initiation propagation of GNA largely GABA-independent dominated glutamatergic transmission via AMPA receptors. Despite fact that signal adult hippocampus strongly unidirectional through canonical trisynaptic circuit (dentate gyrus [DG] CA1), originates distal propagates both forward CA1 backward DG. Photostimulation-evoked also shows prominent Mouse correlated electrophysiological highly localized local field potential events. Photostimulation mapping circuitry demonstrates enhancement connections excitatory neurons occurs over same time course as reveals underlying pathways accounting for disappearance coincides transition adult-like organization at about 2 weeks age. Taken together, our findings suggest critical link between maturation functional hippocampus.