Redefining the boundaries of the hippocampal CA2 subfield in the mouse using gene expression and 3-dimensional reconstruction

摘要: The morphology of neurons in the main divisions hippocampal complex allow easy identification granule cells dentate gyrus and pyramidal CA1 CA3 regions Ammon's horn. However, CA2 subfield have been much more difficult to reliably identify. We recently identified a set genes whose expression is restricted either gyrus, CA1, CA2, or CA3. Here we show that these an essentially nonoverlapping distribution throughout entire septotemporal extent hippocampus. 3-Dimensional reconstruction serial sections processed for situ hybridization mannosidase 1, alpha (CA1), bcl-2-related ovarian killer protein (CA3), Purkinje cell 4 (dentate + CA2) was used define boundaries each subregion observed three are recapitulated across larger similarly enriched specific subregions. defined on basis gene somewhat than previously described structural anatomical criteria, particularly at rostral pole These results indicate that, least molecular level, there robust, consistent genetic between subregions CA3, allowing redefinition their order facilitate functional studies different neuronal subtypes J. Comp. Neurol. 485:1–10, 2005. © 2005 Wiley-Liss, Inc.