Precise navigation surgery of tumours in the lung in mouse models enabled by in situ fluorescence labelling with a killer-reporter adenovirus.

作者: Shuya Yano , Yong Zhang , Shinji Miwa , Hiroyuki Kishimoto , Yasuo Urata

DOI: 10.1136/BMJRESP-2015-000096

关键词:

摘要: Background Current methods of image-guided surgery tumours the lung mostly rely on CT. A sensitive procedure selective tumour fluorescence labelling would allow simple and high-resolution visualisation for precise surgical navigation. Methods Human cancer cell lines H460 A549 were genetically transformed to express red fluorescent protein (RFP). Tumours grown subcutaneously each line harvested minced orthotopic implantation left nude mice. Tumour growth was measured by imaging. After reached 5 mm in diameter, they injected under guidance with telomerase-dependent green (GFP)-containing adenovirus, OBP-401. Viral GFP precisely colocalised RFP expression. Three days after administration OBP-401, fluorescence-guided (FGS) performed. Results FGS enabled a adenovirus containing gene. selectively brightly labelled. complete resection no residual tumour. Conclusions is feasible more effective than bright-light surgery.

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