Synergistic activity of luteolin and amoxicillin combination against amoxicillin-resistant Escherichia coli and mode of action.
作者： G. Eumkeb , S. Siriwong , K. Thumanu
DOI: 10.1016/J.JPHOTOBIOL.2012.10.006
关键词:
摘要: The purpose of this research was to investigate whether luteolin has antibacterial and synergistic activity against amoxicillin-resistant Escherichia coli (AREC) when use singly in combination with amoxicillin. primarily mode action is also investigated. susceptibility assay (minimum inhibitory concentration checkerboard determination) carried out by the broth macrodilution method's Mueller-Hinton medium. MIC determination were after 20 h incubation at 35°C observing turbidity. MICs amoxicillin all AREC strains >1000 ≥ 200 μg/ml respectively. Synergistic observed on plus these strains. Viable count showed effect reducing cell numbers. results indicated that altered both outer inner membrane permeabilisation. Enzyme had an penicillinase. Fourier Transform-Infrared (FT-IR) spectroscopy exhibited alone combined caused increase fatty acid nucleic acid, but decrease amide I proteins bacterial envelops compared control. These potential reverse resistance may operate via three mechanisms: inhibition peptidoglycan synthesis, certain extended-spectrum β-lactamases alteration permeability. findings offer develop a new generation phytopharmaceuticals treat AREC.
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