Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.

摘要: Cyclin E is classified as a putative G1 cyclin on the basis of its cyclic pattern mRNA expression, with maximal levels being detected near G1/S boundary. We report here that found associated transcription factor E2F in temporally regulated fashion. known to be critical for expression some S phase-specific proteins and thought important series others. Antisera specific were raised used demonstrate an association between E2F. This E/E2F complex was seen variety human cell lines from various tissues, but appearance primarily during phase cycle. The decreased cells entered phase, just A became detectable. characterized E-E2F further show both cyclin-dependent kinase-2 (cdk2) p107 present. Therefore, p107/E2F two different cdk2 kinase complexes--one containing other E--and these complexes presence suggests role control genes required G1-to-S transition.