A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures.
作者： Gerald Cohen , Mimi Kim , Vivian Ogwu
DOI: 10.1016/0165-0270(96)00011-8
关键词:
摘要: The enzyme catalase is present in relatively small amounts neural tissue. A standard spectrophotometric assay for has been modified to make it suitable both automated with a plate reader and the analysis of cell cultures. Catalase activity determined by measuring residual hydrogen peroxide after incubation enzyme. Ferrous ions thiocyanate are used determination peroxide. stable visible absorption ferrithiocyanate can be measured either or spectrophotometer. method assays from single wells tissue culture containing 2-3 mg embryonic rat mesencephalon.
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