Development of a Biosensor-based Immunoassay for Screening of Chloramphenicol Residues in Milk

摘要: Biosensor immunoassays were developed recently for antibiotics with an established maximum residue limit (MRL). In this study, according to the regulatory banning of chloramphenicol (CAP) use food producing animals, main objectives were: specificity biosensor assay and lowest detection possible. The was based on inhibition binding polyclonal antibodies against CAP immobilized a sensor chip by in solution. response varied inversely antibiotic concentration sample. Two different two immobilization protocols tested. As ELISA tests antibody influenced performances. Moreover, we showed that particular care should be concentrated step because it is critical point development. Three milk. best obtained 1 milk base surface its very low (0.1 w g l−1) ...