Thermoresponsive β-glucan-based polymers for bimodal immunoradiotherapy - Are they able to promote the immune system?

摘要: A conceptually new bimodal immunoradiotherapy treatment was demonstrated using thermoresponsive polymer β-glucan-graft-poly(2-isopropyl-2-oxazoline-co-2-butyl-2-oxazoline) bearing complexes of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid with yttrium-90(III) at the graft ends. The behavior this in aqueous solutions studied, and it showed appropriate cloud point temperature for brachytherapy applications. tested vitro, exhibited nontoxicity active uptake into cancer cells macrophages colocalization lysosomes macrophagosomes. Moreover, observed oxidative burst response leukocytes established immunostimulatory properties polymer, which were also studied vivo after injection thigh muscles healthy mice. subsequent histological evaluation revealed extensive immune activation reactions site injection. Furthermore, production tumor necrosis factor α induced by prepared denoting optimistic prognosis treatment. biodistribution study indicated formation depot, gradually degraded excluded from body. radiolabeled used during antitumor efficiency experiments on mice EL4 lymphoma. group (treated polymer) complete inhibition growth beginning 7 15 completely cured group, while others significantly prolonged survival time compared to control group. considerable synergistic effect separately immunotherapy or radiotherapy.