Heme Oxygenase 1-Mediated Neurogenesis Is Enhanced by Ginkgo biloba (EGb 761®) After Permanent Ischemic Stroke in Mice

摘要: Stroke is the fourth leading cause of death and a major disability in stroke survivors. Studies have underlined importance repair mechanisms recovery phase stroke. Neurogenesis response to brain injury one regeneration processes that, if enhanced, may offer better treatment alternatives. Previously, we demonstrated antioxidant, neuritogenic, angiogenic properties Ginkgo biloba/EGb 761® (EGb 761) different mouse models In present study, were interested study whether EGb 761 could protect mice from permanent middle cerebral artery occlusion (pMCAO) enhance neurogenesis. pre- posttreated had lower infarct volume improved motor skills with enhanced proliferation neuronal stem/progenitor cells (NSPCs) at 24 h 7 days posttreatment. Netrin-1 its receptors (DCC UNC5B) that mediate axonal attraction repulsion observed be overexpressed NSPCs only, implying netrin-1 might partly played role Interestingly, heme oxygenase 1 knockout (HO1−/−), neurogenesis was significantly than vehicle-treated day 8. Furthermore, also (HO1)-activated pathway phosphorylated glycogen synthase kinase 3 α/β (p-GSK-3 α/β), collapsin mediator protein 2 (CRMP-2), semaphorin3A (SEMA3A), Wnt, suggesting probable signaling pathways involved proliferation, differentiation, migration NSPCs. Together, these results propose not only has properties, but can augment following