Tissue microarray molecular profiling of early, node-negative adenocarcinoma of the rectum: a comprehensive analysis.

摘要: Purpose: Early-stage adenocarcinoma of the rectum treated with curative intent has a favorable overall prognosis; however, 20%–30% patients recur, and majority ultimately die disease. Recurrence tumor-related mortality may be attributable to molecular abnormalities in primary tumors accounting for their more aggressive biological behavior. This study evaluates such phenotypes regard cell cycle regulation proliferation determines significance patient outcome. Experimental Design: One hundred T 2–3 , N 0 uniformly by surgery alone were studied. Core biopsies pathological specimens assembled on tissue microarrays, expression p53, mdm-2, p21, Bcl-2, p27, cyclin D1, Ki-67 was analyzed immunohistochemistry. Molecular profiles correlated disease-free (DFS) disease-specific survival (DSS). Results: Despite previously described prognostic relevance some investigated molecules analyses where different stages colorectal cancer included, none cycle-regulatory or proliferation-related markers associated recurrence survival. However, demonstrating down-regulation cyclin-dependent kinase inhibitor tumor suppressor gene development metastases, showed trend toward reduced DFS DSS ( P = 0.06 0.07, respectively). Conclusions: In this homogeneous group early-stage, node-negative alone, proliferation-associated proteins appear have no significance. p27 appears DSS, which suggests further investigation other p27-related pathways potentially relevant metastatic