microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

摘要: microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to aberrant expression in a number tumor types. miR-192, -194 and -215 not comprehensively investigated using large cases colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues three cell lines. Following polyadenylation reverse transcription, the levels were determined for evaluation association between clinicopathological characteristics by quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied impact miR-194 on proliferation HCT-116 cells MTT assay. significantly downregulated (all p<0.001, paired t-test) lines p<0.05) compared non-tumor counterparts. Moreover, demonstrated be associated with increased sizes (p=0.027, p=0.018, p=0.027, respectively; Mann-Whitney U test). Also, there marked correlations among these miRNAs Pearson's regression analysis). Furthermore, found that overexpression could inhibit HTC-116 cells. may important biological markers as suppressors carcinogenesis CRC.