Two rapd-scar markers in new gift Nile tilapia (Oreochromis niloticus) implication for selection tracking and strains identification.
作者： Tang ShouJie , H. Elghobashy , Li SiFa , K. Fitzsimmons , A. S. Diab
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摘要: The NEW GIFT Nile tilapia is a national certificated new strain produced through 14 years and 9 generations selection from the base of tilapia. From two strain-specific RAPD bands namely S304 S36 were identified amplified 50 80 10bp-oligo-nucleotide random primers separately after gel extraction cloning sequencing pairs primer(20 for forward primer 21 reverse primer) was designed according to sequence information, then PCR amplification carried out in strains other 7 farmed strains. For first primer, one 553bp SCAR showed 86.7% appearing frequency but only 17% strain. This marker has been used as specific monitor tracking selection. second 558bp 91.4% 0-70% molecular identification development these markers indicated that some alleles related with economically important traits might increase during
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