Postischemic cell death in reperfused porcine hearts is not attenuated by the spin trap agent PBN during early reperfusion.
作者： K. Nebendahl , J. Schaper , C. Schadebrittinger , S. Lindertheimberg , S. Pich
DOI: 10.1007/BF00794994
关键词:
摘要: Ischemic, reperfused porcine hearts were used to investigate whether the spin trap agent PBN (N-tert-butyl-alpha-phenylnitrone) attenuates postischemic cell death by scavenging of free radicals. The left anterior descending coronary artery (LAD) was ligated distally in 16 pigs for 45 min and then 3 h. (coronary concentration approximately 1 mM) infused into LAD eight during first reperfusion. Electron resonance spectroscopy (ESR) performed identify radical adducts venous blood. Regional systolic shortening (SS%) determined sonomicrometry. Infarct size evaluated as percentage infarcted (tetrazolium stain) ischemic (dye technique) myocardium. transmural ultrastructural degree myocardial injury well ATP levels assessed at end experiment.
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D. Baller, H. J. Bretschneider, G. Hellige, Validity of myocardial oxygen consumption parameters. Clinical Cardiology. ,vol. 2, pp. 317- 327 ,(1979) , 10.1002/CLC.4960020502
Theodor K. Shnitka, Marvin M. Nachlas, Macroscopic identification of early myocardial infarcts by alterations in dehydrogenase activity. American Journal of Pathology. ,vol. 42, pp. 379- 405 ,(1963)
G L Freeman, M M LeWinter, R L Engler, J W Covell, Relationship between myocardial fiber direction and segment shortening in the midwall of the canine left ventricle. Circulation Research. ,vol. 56, pp. 31- 39 ,(1985) , 10.1161/01.RES.56.1.31
H Klein, Failure of iloprost to protect the regionally ischemic, reperfused porcine heart. Journal of Molecular and Cellular Cardiology. ,vol. 23, pp. 963- 972 ,(1991) , 10.1016/0022-2828(91)90138-C
Benedict Robert Lucchesi, Steven W Werns, Joseph C Fantone, The role of the neutrophil and free radicals in ischemic myocardial injury Journal of Molecular and Cellular Cardiology. ,vol. 21, pp. 1241- 1251 ,(1989) , 10.1016/0022-2828(89)90670-6
D J Hearse, A Tosaki, Free radicals and reperfusion-induced arrhythmias: protection by spin trap agent PBN in the rat heart Circulation Research. ,vol. 60, pp. 375- 383 ,(1987) , 10.1161/01.RES.60.3.375
H. H. Klein, S. Pich, K. Nebendahl, P. Niedmann, P. Schuff-Werner, Acute treatment with vitamin E does not protect the regionally ischemic, reperfused porcine heart Basic Research in Cardiology. ,vol. 86, pp. 32- 39 ,(1991) , 10.1007/BF02193869
J Schaper, Ultrastructural, functional, and biochemical criteria for estimation of reversibility of ischemic injury: a study on the effects of global ischemia on the isolated dog heart. Journal of Molecular and Cellular Cardiology. ,vol. 11, pp. 521- 528 ,(1979) , 10.1016/0022-2828(79)90428-0
R. Bolli, M. O. Jeroudi, B. S. Patel, C. M. DuBose, E. K. Lai, R. Roberts, P. B. McCay, Direct evidence that oxygen-derived free radicals contribute to postischemic myocardial dysfunction in the intact dog Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 4695- 4699 ,(1989) , 10.1073/PNAS.86.12.4695
G W Mergner, W B Weglicki, J H Kramer, Postischemic free radical production in the venous blood of the regionally ischemic swine heart. Effect of deferoxamine. Circulation. ,vol. 84, pp. 2079- 2090 ,(1991) , 10.1161/01.CIR.84.5.2079