ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6
作者： Thomas O. Eichmann , Lukas Grumet , Ulrike Taschler , Jürgen Hartler , Christoph Heier
DOI: 10.1194/JLR.M062372
关键词:
摘要: Lipid droplets (LDs) of hepatic stellate cells (HSCs) contain large amounts vitamin A [in the form retinyl esters (REs)] as well other neutral lipids such TGs. During times insufficient availability, RE stores are mobilized to ensure a constant supply body. To date, little is known about enzymes responsible for hydrolysis lipid esters, in particular REs, HSCs. In this study, we aimed identify LD-associated hydrolases by proteomic approach using rat cell line HSC-T6. First, loaded with retinol and FAs promote synthesis deposition within LDs. Then, LDs were isolated composition LD proteome analyzed. Among proteins, found perilipin 2, adipose TG lipase (ATGL), comparative gene identification-58 (CGI-58), established proteins. Bioinformatic search α/β-hydrolase fold-containing proteins revealed no yet uncharacterized hydrolases. vitro activity assays, show that (r)ATGL, coactivated (r)CGI-58, efficiently hydrolyzes TGs REs. These findings suggest rATGL rCGI-58 LD-resident HSCs participate mobilization both REs
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