Transformation of primary rat kidney cells by fragments of simian virus 40 DNA.
作者： P J Abrahams , C Mulder , A Van De Voorde , S O Warnaar , A J van der Eb
DOI: 10.1128/JVI.16.4.818-823.1975
关键词:
摘要: Abstract Linear simian virus 40 (SV40) DNA molecules of genome length and fragments smaller than when prepared with restriction endonucleases tested for transforming activity on primary cultures baby rat kidney cells. The linear (prepared R-EcoRI, R-BamHI, R-HpaII or R-HapII), a 74% fragment (EcoRI/HpaII HapII-A), 59% (BamHI/HapII-A) could all transform cells the same efficiency as circular SV40 DNA. All transformed lines contained SV40-specific T-antigen in 90 to 100% cells, which was taken evidence that transformation specific. entire early region Endo R-HpaI, introduced one break region, apparently inactivated capacity DNA, since no observed any three HpaI tested. Attempts were made rescue infectious from some by fusion permissive BSC-1 Infectious only recovered form I No be isolated other types
sci-hub.st 参考文章(28)
J.F. Watkins, Fusion of Cells for Virus Studies and Production of Cell Hybrids Methods in Virology. ,vol. 5, pp. 1- 32 ,(1971) , 10.1016/B978-0-12-470205-9.50007-1
J S Brugge, J S Butel, Role of simian virus 40 gene A function in maintenance of transformation. Journal of Virology. ,vol. 15, pp. 619- 635 ,(1975) , 10.1128/JVI.15.3.619-635.1975
George C. Fareed, Claude F. Garon, Norman P. Salzman, Origin and Direction of Simian Virus 40 Deoxyribonucleic Acid Replication Journal of Virology. ,vol. 10, pp. 484- 491 ,(1972) , 10.1128/JVI.10.3.484-491.1972
Kathleen J. Danna, George H. Sack, Daniel Nathans, Studies of Simian virus 40 DNA Journal of Molecular Biology. ,vol. 78, pp. 363- 376 ,(1973) , 10.1016/0022-2836(73)90122-8
P Tegtmeyer, Function of simian virus 40 gene A in transforming infection. Journal of Virology. ,vol. 15, pp. 613- 618 ,(1975) , 10.1128/JVI.15.3.613-618.1975
R G Martin, J Y Chou, Simian virus 40 functions required for the establishment and maintenance of malignant transformation. Journal of Virology. ,vol. 15, pp. 599- 612 ,(1975) , 10.1128/JVI.15.3.599-612.1975
P. J. Abrahams, A. J. van der Eb, In Vitro Transformation of Rat and Mouse Cells by DNA from Simian Virus 40 Journal of Virology. ,vol. 16, pp. 206- 209 ,(1975) , 10.1128/JVI.16.1.206-209.1975
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
Bernhard Hirt, Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology. ,vol. 26, pp. 365- 369 ,(1967) , 10.1016/0022-2836(67)90307-5
C. Mulder, J. R. Arrand, H. Delius, W. Keller, U. Pettersson, R. J. Roberts, P. A. Sharp, Cleavage Maps of DNA from Adenovirus Types 2 and 5 by Restriction Endonucleases EcoRI and HpaI Cold Spring Harbor Symposia on Quantitative Biology. ,vol. 39, pp. 397- 400 ,(1974) , 10.1101/SQB.1974.039.01.051