An H3K36 Methylation-Engaging Tudor Motif of Polycomb-like Proteins Mediates PRC2 Complex Targeting
作者： Ling Cai , Scott B. Rothbart , Rui Lu , Bowen Xu , Wei-Yi Chen
DOI: 10.1016/J.MOLCEL.2012.11.026
关键词:
摘要: Polycomb repressive complex 2 (PRC2) regulates pluripotency, differentiation, and tumorigenesis through catalysis of histone H3 lysine 27 trimethylation (H3K27me3) on chromatin. However, the mechanisms that underlie PRC2 recruitment spreading chromatin remain unclear. Here we report 36 (H3K36me3) binding activity is harbored in Tudor motifs PRC2-associated polycomb-like (PCL) proteins PHF1/PCL1 PHF19/PCL3. Ectopically expressed PHF1 induced Tudor-dependent stabilization complexes bulk mediated H3K27me3 into H3K36me3-containing regions. In murine pluripotent stem cells, identified coexistence H3K36me3, H3K27me3, PHF19/PCL3 at a subset poised developmental genes demonstrated function required for optimal repression these loci. Collectively, our data suggest PCL recognition H3K36me3 promotes intrusion active regions to promote gene silencing modulate landscape during development.
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