Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms.

摘要: The tube-within-tube body plan of earthworms is appropriate for studying the interactions microorganisms with immune system cavities such as digestive tract and coelom. This study aims to describe response on molecular cellular level in coelomic cavity gut earthworm Eisenia andrei after experimental microbial challenge by administering two bacterial strains (Escherichia coli Bacillus subtilis) or yeast Saccharomyces cerevisiae environment. changes mRNA levels defense molecules (pattern recognition receptor CCF, lysozyme, fetidin/lysenins) coelomocytes tissue were determined quantitative PCR. at a was captured histological sections, expression CCF localized using situ hybridization. Coelomocytes respond presence bacteria increasing molecules, especially CCF. less affected stimulation because epithelial cells exhibit basically strong synthesis ccf against continuous load lumen. mediated released from mesenchymal lining cavity. These combined mechanisms are necessary survival microbially rich environment soil.