Kinetic characterization of the deproteinization of trabecular and cortical bovine femur bones.

作者: Ana B. Castro-Ceseña , M. Pilar Sánchez-Saavedra , Ekaterina E. Novitskaya , Po-Yu Chen , Gustavo A. Hirata

DOI: 10.1016/J.MSEC.2013.08.022

关键词:

摘要: The present study proposes an interpretation of the mechanism bone deproteinization. Cortical and trabecular bovine femur bones were deproteinized using 6% NaOCl (37, 50, 60°C). kinetic parameters (rate constant activation energy) calculated, surface area each type was considered. A statistical analysis rate constants shows that cortical deproteinizes at a lower than trabecular. energy is higher for bone, no significant differences are found in protein concentration values both bones. Therefore, although cortical, requires more deproteinization reaction to take place. Considering types constituted by mineral, protein, water; work individual inner matrix architecture bones, along with characteristics such as mineral its bonding collagen fibers, may be responsible factors control depletion.

参考文章(26)
J. J BROZ, S. J SIMSKE, W. D CORLEY, A. R GREENBERG, Effects of deproteinization and ashing on site-specific properties of cortical bone Journal of Materials Science: Materials in Medicine. ,vol. 8, pp. 395- 401 ,(1997) , 10.1023/A:1018545303184
Leon Weiss, Histology : cell and tissue biology Elsevier Biomedical. ,(1983)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Arne Mordenfeld, Mats Hallman, Carina B. Johansson, Tomas Albrektsson, Histological and histomorphometrical analyses of biopsies harvested 11 years after maxillary sinus floor augmentation with deproteinized bovine and autogenous bone. Clinical Oral Implants Research. ,vol. 21, pp. 961- 970 ,(2010) , 10.1111/J.1600-0501.2010.01939.X
Aline Percot, Christophe Viton, Alain Domard, Characterization of shrimp shell deproteinization Biomacromolecules. ,vol. 4, pp. 1380- 1385 ,(2003) , 10.1021/BM034115H
N Pleshko Camacho, S Rinnerthaler, EP Paschalis, R Mendelsohn, AL Boskey, P Fratzl, Complementary information on bone ultrastructure from scanning small angle X-ray scattering and Fourier-transform infrared microspectroscopy Bone. ,vol. 25, pp. 287- 293 ,(1999) , 10.1016/S8756-3282(99)00165-9
Philipp J. Thurner, Blake Erickson, Patricia Turner, Ralf Jungmann, Jason Lelujian, Alexander Proctor, James C. Weaver, Georg Schitter, Daniel E. Morse, Paul K. Hansma, The Effect of NaF In Vitro on the Mechanical and Material Properties of Trabecular and Cortical Bone Advanced Materials. ,vol. 21, pp. 451- 457 ,(2009) , 10.1002/ADMA.200801204
A.B. Castro-Ceseña, E.E. Novitskaya, P.-Y. Chen, G.A. Hirata, J. McKittrick, Kinetic studies of bone demineralization at different HCl concentrations and temperatures Materials Science and Engineering: C. ,vol. 31, pp. 523- 530 ,(2011) , 10.1016/J.MSEC.2010.11.003
D. Carter, W. Hayes, Bone compressive strength: the influence of density and strain rate Science. ,vol. 194, pp. 1174- 1176 ,(1976) , 10.1126/SCIENCE.996549