Co-transformation with autonomous replicating and integrative plasmids in Penicillium chrysogenum is highly efficient and leads in some cases to rescue of the intact integrative plasmid.
作者： Oscar Bañuelos , Leopoldo Naranjo , Javier Casqueiro , Santiago Gutiérrez , Juan F Martı́n
DOI: 10.1016/S1087-1845(03)00081-1
关键词:
摘要: Abstract The efficiency of co-transformation in Penicillium chrysogenum Wisconsin 54-1255 pyrG − and the fate transforming DNA were studied using an integrative (pEF43) autonomous replicating plasmid (pAM9L). results showed a frequency nearly 70% all transformants tested. total transformation was shown to be dependent on marker used as transformant selection (i.e., markers or vector). Analysis plasmids re-isolated from several co-transformants that different populations co-exist fungal host. Interestingly, studied, found autonomously without integrating into host genome. In some cases, co-integrates formed by recombination between (pAM9L) plasmids. However, unexpectedly non-reorganised pEF43 assays rescued co-transformants.
elsevier.com
sci-hub.se 参考文章(39)
Juan F. Martín, Javier Casqueiro, Katarina Kosalková, Ana T. Marcos, AFF1>Santiago Gutiérrez, Penicillin and cephalosporin biosynthesis: mechanism of carbon catabolite regulation of penicillin production. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 75, pp. 21- 31 ,(1999) , 10.1023/A:1001820109140
B Díez, S Gutiérrez, J L Barredo, P van Solingen, L H van der Voort, J F Martín, The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes. Journal of Biological Chemistry. ,vol. 265, pp. 16358- 16365 ,(1990) , 10.1016/S0021-9258(17)46231-4
V W Yang, J A Marks, B P Davis, T W Jeffries, High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Applied and Environmental Microbiology. ,vol. 60, pp. 4245- 4254 ,(1994) , 10.1128/AEM.60.12.4245-4254.1994
F J Kretschmer, A C Chang, S N Cohen, Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. Journal of Bacteriology. ,vol. 124, pp. 225- 231 ,(1975) , 10.1128/JB.124.1.225-231.1975
K Sakai, J Sakaguchi, M Yamamoto, High-frequency cotransformation by copolymerization of plasmids in the fission yeast Schizosaccharomyces pombe. Molecular and Cellular Biology. ,vol. 4, pp. 651- 656 ,(1984) , 10.1128/MCB.4.4.651
M B Kurtz, M W Cortelyou, S M Miller, M Lai, D R Kirsch, Development of autonomously replicating plasmids for Candida albicans. Molecular and Cellular Biology. ,vol. 7, pp. 209- 217 ,(1987) , 10.1128/MCB.7.1.209
P. Fournier, A. Abbas, M. Chasles, B. Kudla, D. M. Ogrydziak, D. Yaver, J. W. Xuan, A. Peito, A. M. Ribet, C. Feynerol, Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 4912- 4916 ,(1993) , 10.1073/PNAS.90.11.4912
David Gems, Iain L. Johnstone, A.John Clutterbuck, An Autonomously Replicating Plasmid Transforms Aspergillus Nidulans at High Frequency Gene. ,vol. 98, pp. 61- 67 ,(1991) , 10.1016/0378-1119(91)90104-J
J. Casqueiro, O. Bañuelos, S. Gutiérrez, M. J. Hijarrubia, J. F. Martín, Intrachromosomal recombination between direct repeats in Penicillium chrysogenum : gene conversion and deletion events Molecular Genetics and Genomics. ,vol. 261, pp. 994- 1000 ,(1999) , 10.1007/S004380051048
Francisco Fierro, Santiago Gutiérrez, Bruno Diez, Juan F. Martín, Resolution of four large chromosomes in penicillin-producing filamentous fungi: the penicillin gene cluster is located on chromosome II (9.6 Mb) in Penicillium notatum and chromosome 1 (10.4 Mb) in Penicillium chrysogenum Molecular and General Genetics MGG. ,vol. 241-241, pp. 573- 578 ,(1993) , 10.1007/BF00279899