Human testicular aromatase: immunocytochemical and biochemical studies

摘要: The location of the aromatase enzyme in testes human subjects ages 3 months to 72 yr was determined immunocytochemically using a monoclonal antibody specific aromatase. In addition, an assay developed measure activity suspensions cryosections order validate presence and quantitate sections. mature testes, immunostain always associated with Leydig cells absent from Sertoli cells. Aromatase ranged 0.014-0.55 pmol estrogen per mg/h significantly correlated intensity (P < 0.02). Thus, provided semiquantitative estimate immunoreaction. Activity did not correlate increasing age. Rather, highest levels were measured four six men 18-20 yr, three whom also had strongest for cell clusters these larger more prominent than those other specimens. A low level but no detected prepubertal testes. by either method normal tissue testis patient testicular cancer.