Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation
作者： Anne Wiesmann , Hans-Jörg Bühring , Christoph Mentrup , Hans-Peter Wiesmann
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摘要: Mesenchymal stem cells (MSC) are multipotent which can differentiate along osteogenic, chondrogenic, and adipogenic lineages. The present study was designed to investigate the influence of mechanical force as a specific physiological stress on differentiation osteoblast-like cells. Human MSC were cultured in osteoinductive medium with or without cyclic uniaxial stimulation (2000 μstrain, 200 cycles per day, 1 Hz). Cultured analysed for expression collagen type I, osteocalcin, osteonectin, CD90. To evaluate biomineral formation content bound calcium cultures determined. After 14 days culture immunfluorescence staining revealed enhancement I osteonectin response stimulation. In contrast, mechanically stimulated stained negative unstimulated an increase over time observed. 21 significantly higher compared control cultures. These results demonstrate human into vitro. While significant by is not detected before days, effects extracellular matrix became already obvious after days. decrease CD90 suggests that only transiently expressed during culture.
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