Differential effects of short-chain fatty acids on proliferation and production of pro- and anti-inflammatory cytokines by cultured lymphocytes

摘要: Abstract Short-chain fatty acids (SCFA) are produced by fermentation of water-soluble fiber anaerobic bacteria in the large bowel. Fiber-rich diets decrease risk developing inflammatory bowel disease (IBD) and butyrate enemas effective as a therapy some patients. Crohn's disease, one form IBD, appears to involve an exagerated T helper-1 (Th1) lymphocyte phenotype, characterised production interleukin (IL)-2 interferon (IFN)-γ, that drives inflammation. To examine whether SCFA influence pro- anti-inflammatory cytokine production, rat mesenteric lymph node lymphocytes were cultured presence acetate (10 mM), (1.5 mM) or propionate (2 cytokines response concanavalin A determined. Butyrate, but not propionate, inhibited proliferation IL-2 production. Acetate able partly prevent inhibitory effect on increased IFN-γ whereas it. combination IL-4 was detected any cultures. IL-10 which affected butyrate. It is concluded significantly inhibits Th1-type responses this might explain therapeutic IBD have less marked modulatory actions, cases effects oppose those three causes shift helper phenotype towards more protective fiber.