Modeling and Optimizing Medium Composition for Shoot Regeneration of Chrysanthemum via Radial Basis Function-Non-dominated Sorting Genetic Algorithm-II (RBF-NSGAII)

作者: Mohsen Hesami , Roohangiz Naderi , Masoud Tohidfar

DOI: 10.1038/S41598-019-54257-0

关键词:

摘要: The aim of the current study was modeling and optimizing medium compositions for shoot proliferation chrysanthemum, as a case study, through radial basis function- non-dominated sorting genetic algorithm-II (RBF-NSGAII). RBF one artificial neural networks (ANNs) used four outputs including rate (PR), number (SN), length (SL), basal callus weight (BCW) based on variables 6-benzylaminopurine (BAP), indole-3-butyric acid (IBA), phloroglucinol (PG), sucrose. Afterward, models were linked to optimization algorithm. Also, sensitivity analysis applied evaluating importance each input. R2 correlation values 0.88, 0.91, 0.97, 0.76 between observed predicted data obtained PR, SN, SL, BCW, respectively. According RBF-NSGAII, optimal PR (98.85%), SN (13.32), SL (4.83 cm), BCW (0.08 g) can be from containing 2.16 µM BAP, 0.14 µM IBA, 0.29 mM PG, 87.63 mM results indicated that more sensitive followed by sucrose, IBA. Finally, performance optimized tested, showed difference validation RBF-NSGAII negligible. Generally, considered an efficient computational strategy in vitro organogenesis.

参考文章(44)
Debabrata Sarkar, Prakash S. Naik, Phloroglucinol enhances growth and rate of axillary shoot proliferation in potato shoot tip cultures in vitro Plant Cell Tissue and Organ Culture. ,vol. 60, pp. 139- 149 ,(2000) , 10.1023/A:1006419805980
Viiay Kaul, Robyn M. Miller, James F. Hutchinson, Dennis Richards, Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.) Plant Cell Tissue and Organ Culture. ,vol. 21, pp. 21- 30 ,(1990) , 10.1007/BF00034487
Priyanka Siwach, Anita Rani Gill, Enhanced shoot multiplication in Ficus religiosa L. in the presence of adenine sulphate, glutamine and phloroglucinol Physiology and Molecular Biology of Plants. ,vol. 17, pp. 271- 280 ,(2011) , 10.1007/S12298-011-0074-6
Jorge Gago, Lourdes Martínez-Núñez, Mariana Landin, Jaume Flexas, Pedro P Gallego, None, Modeling the effects of light and sucrose on in vitro propagated plants: a multiscale system analysis using artificial intelligence technology. PLOS ONE. ,vol. 9, ,(2014) , 10.1371/JOURNAL.PONE.0085989
Jorge Gago, Olaya Pérez-Tornero, Mariana Landín, Lorenzo Burgos, Pedro P Gallego, None, Improving knowledge of plant tissue culture and media formulation by neurofuzzy logic: a practical case of data mining using apricot databases. Journal of Plant Physiology. ,vol. 168, pp. 1858- 1865 ,(2011) , 10.1016/J.JPLPH.2011.04.008
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
Yunde Zhao, The role of local biosynthesis of auxin and cytokinin in plant development. Current Opinion in Plant Biology. ,vol. 11, pp. 16- 22 ,(2008) , 10.1016/J.PBI.2007.10.008