Economic laboratory practice in preparative column high-performance liquid chromatography

摘要: Abstract For isolating from 1 mg up to 200 g of pure substance unknown and complex mixtures, a set materials methods is presented evaluated by several applications. The procedure combines automated sequential high-performance liquid chromatography with gradient elution on 50 mm x 21.4 mm, 3-μm packed column. method development time very short the chromatographic system has an high specific production rate. preparative performance discussed, compared other approaches.