Preparative high-performance liquid chromatography under gradient conditions : II. A computer program for the design of reversed-phase gradient-elution separations of peptide and protein samples

摘要: Abstract A commercially available computer program (BIOPREP) is described as an aid for developing preparative separations of peptide or protein samples using reversed-phase gradient elution. On the basis four small-scale runs in laboratory (with advice offered by computer), experimental conditions “touching-band” can be predicted. This turn allows comparisons production rate a purified product function and column dimensions. In this way, selected that either maximize provide otherwise satisfactory separation.