Determination of glycerol in plasma by an automated enzymatic spectrophotometric procedure.
作者： Erik Gerlo
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摘要: The plasma concentration of glycerol, the backbone triglycerides and end product triacylglycerol breakdown, is considered to reflect lipolysis in adipose tissue. We evaluated an automated enzymatic procedure for measurement glycerol plasma. assay was linear up 250 micromol/l. detection limit 8 Recovery averaged 94% from spiked 104% diluted An extensive precision study performed according guidelines National Committee Clinical Laboratory Standards showed within-run coefficients variation between 14.8% 2.6% concentrations ranging 28 micromol/l 164 reference range fasting healthy adult men 14-69 Glycerol levels were significantly correlated with free fatty acid levels. This method rapid reliable, provides greater sensitivity or convenience than previously described procedures.
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