Carbapenem-resistant strain of Klebsiella oxytoca harboring carbapenem-hydrolyzing beta-lactamase KPC-2.
作者： Hesna Yigit , Anne Marie Queenan , J. Kamile Rasheed , James W. Biddle , Antonio Domenech-Sanchez
DOI: 10.1128/AAC.47.12.3881-3889.2003
关键词:
摘要: We investigated a Klebsiella oxytoca isolate demonstrating resistance to imipenem, meropenem, extended-spectrum cephalosporins, and aztreonam. The MICs of both imipenem meropenem were 32 microg/ml. beta-lactamase activity against was inhibited in the presence clavulanic acid. Isoelectric focusing studies demonstrated five beta-lactamases with pIs 8.2 (SHV-46), 6.7 (KPC-2), 6.5 (unknown), 6.4 (probable OXY-2), 5.4 (TEM-1). bla(SHV) bla(TEM) genes confirmed by specific PCR assays DNA sequence analysis. Transformation conjugation Escherichia coli showed that pI 6.7, pneumoniae carbapenemase-2 encoded on an approximately 70-kb conjugative plasmid also carried SHV-46, TEM-1, 6.5. bla(KPC-2) determinant cloned E. conferred amino acid KPC-2 single difference, S174G, when compared KPC-1, another carbapenem-hydrolyzing from K. 1534. Hydrolysis purified hydrolyzed not only carbapenems but penicillins, had highest affinity for meropenem. kinetic revealed tazobactam. An examination outer membrane proteins parent strain it expressed detectable levels OmpK36 (the homolog OmpC) higher-molecular-weight OmpK35 OmpF). Thus, carbapenem 3127 is due production Bush group 2f, class A, KPC-2. This likely located transposon part thus has very high potential dissemination.
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