Purification and characterization of Pseudomonas aeruginosa lipase produced by SSF of deoiled Jatropha seed cake
作者： Chetna Joshi , S.K. Khare
DOI: 10.1016/J.BCAB.2012.08.006
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摘要: Abstract A solvent stable lipase from Pseudomonas aeruginosa PseA is described. The was produced by solid-state fermentation of deoiled Jatropha seed cake and purified ultrafiltration gel filtration chromatography using Sephadex G-100. characterized for its enzymatic properties stability. It exhibited remarkable stability in a wide range organic solvents up to 75% (v/v) concentrations. Circular dichroism intrinsic fluorescence spectra were recorded investigate the effect on secondary tertiary structures. structure predominantly α-helical which remained unperturbed even at high concentration after 24 h exposure. Highly hydrophobic tetradecane caused loss activity lipase. exploited an efficient synthesis ethyl butyrate n-hexane medium.
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