Simple, fast and high-throughput single-cell analysis on PDMS microfluidic chips
作者： Linfen Yu , Huaiqing Huang , Xiuling Dong , Dapeng Wu , Jinhua Qin
关键词:
摘要: this paper demonstrated the chemical analysis of single cell on a cross pdms microfluidic chip in simple, fast, and high-throughput mode. pre-stained cells were sequentially loaded into section by hydrodynamic force, lysed 0.2% sds subsequently lysates detected lif. each can be within 500 ms due to its high concentration at resulted from absence electro-osmosis after surface coating microchannel. reliability quality was confirmed glutathione rhodamine 123 k562 cells. run, approximately 100 could analyzed about 10 min, which comparatively throughput. proposed method is throughput, might significance identifying biological molecules involved fast biochemical processes studying heterogenous
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Gavin D. Meredith, Christopher E. Sims, Joseph S. Soughayer, Nancy L. Allbritton, Measurement of kinase activation in single mammalian cells. Nature Biotechnology. ,vol. 18, pp. 309- 312 ,(2000) , 10.1038/73760
Ricardo E. Dolmetsch, Keli Xu, Richard S. Lewis, Calcium oscillations increase the efficiency and specificity of gene expression Nature. ,vol. 392, pp. 933- 936 ,(1998) , 10.1038/31960
Maxine A. McClain, Christopher T. Culbertson, Stephen C. Jacobson, Nancy L. Allbritton, Christopher E. Sims, J. Michael Ramsey, Microfluidic Devices for the High-Throughput Chemical Analysis of Cells Analytical Chemistry. ,vol. 75, pp. 5646- 5655 ,(2003) , 10.1021/AC0346510
Sergey N. Krylov, Dieter A. Starke, Edgar A. Arriaga, Zheru Zhang, Nora W. C. Chan, Monica M. Palcic, Norman J. Dovichi, Instrumentation for chemical cytometry. Analytical Chemistry. ,vol. 72, pp. 872- 877 ,(2000) , 10.1021/AC991096M
Jianhua Qin, Nannan Ye, Linfen Yu, Dayu Liu, Yingsing Fung, Wei Wang, Xiaojun Ma, Bingcheng Lin, Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis Electrophoresis. ,vol. 26, pp. 1155- 1162 ,(2005) , 10.1002/ELPS.200410136
Thomas P. Roddy, Donald M. Cannon,, Sara G. Ostrowski, Nicholas Winograd, Andrew G. Ewing, Identification of cellular sections with imaging mass spectrometry following freeze fracture. Analytical Chemistry. ,vol. 74, pp. 4020- 4026 ,(2002) , 10.1021/AC025574W
Joseph S. Soughayer, Tatiana Krasieva, Stephen C. Jacobson, J. Michael Ramsey, Bruce J. Tromberg, Nancy L. Allbritton, Characterization of cellular optoporation with distance Analytical Chemistry. ,vol. 72, pp. 1342- 1347 ,(2000) , 10.1021/AC990982U
Torsten Vilkner, Dirk Janasek, Andreas Manz, Micro total analysis systems. Recent developments. Analytical Chemistry. ,vol. 76, pp. 3373- 3385 ,(2004) , 10.1021/AC040063Q
Phillip Belgrader, Derek Hansford, Gregory T. A. Kovacs, Kodumudi Venkateswaran, Raymond Mariella,, Fred Milanovich, Shanavaz Nasarabadi, Margaret Okuzumi, Farzad Pourahmadi, M. Allen Northrup, A Minisonicator To Rapidly Disrupt Bacterial Spores for DNA Analysis Analytical Chemistry. ,vol. 71, pp. 4232- 4236 ,(1999) , 10.1021/AC990347O
Karel Klepárník, Marcel Horký, Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device. Electrophoresis. ,vol. 24, pp. 3778- 3783 ,(2003) , 10.1002/ELPS.200305667