The overexpression of miR-30a affects cell proliferation of chondrosarcoma via targeting Runx2

摘要: MicroRNAs (miRNAs) are emerging as important epigenetic modulators of multiple target genes, leading to abnormal cellular signaling involving proliferation in cancers. Aberrant miRNA expression has been observed human chondrosarcoma (CS). The purpose the present study was evaluate and molecular mechanisms Runx2 miR-30a CS tissues cell lines JJ012, SW1353, L3252. In study, we found that markedly downregulated tissues, compared matched non-tumor-associated tissues. Furthermore, inversely proportional messenger RNA (mRNA) protein. Upregulation dramatically reduced proliferation, colony formation, cycle-related proteins cells. Flow cytometry analysis showed ectopic significantly decreased percentage S phase cells increased G1/G0 Luciferase reporter assays confirmed binding 3'-untranslated region (3'-UTR) inhibited cancer Taken together, our results suggest plays an role inhibit presents a novel mechanism for direct miRNA-mediated suppression CS. Thus, miR-30a/Runx2 may have treatment patients.