Multifunctional biosensors based on peptide–polyelectrolyte conjugates

摘要: A novel enzymatic platform for the sensing of H2O2 and glucose that uses L,L-diphenylalanine micro/nanostructures (FF-MNSs) as an enzyme support is shown. This obtained by self-assembly poly(allylamine hydrochloride) (PAH), FF-MNSs, microperoxidase-11 (MP11) anchored onto peptide matrix, in two different crystal structures FF-MNSs: hexagonal (P61) orthorhombic (P22121). The electroactive area electrodes increases presence FF-MNSs. We also demonstrate via theoretical calculations valence band energy structure allows it to be doped, similarly p-type semiconductors, where PAH acts a doping agent structure, decreasing band-gap around 1 eV, which results smaller charge transfer resistance. These are consistent with electrochemical impedance spectroscopy measurements, further elucidate role FF-MNSs conductivity electron rates hybrid material. An effective communication between electrode active site oxidase through MP11–protein complexes occurs, paving way phase future development bioelectronics devices.