Expression and function of the interleukin 7 receptor in murine lymphocytes

摘要: Abstract A monoclonal antibody, A7R34, that recognizes the high-affinity interleukin 7 receptor (IL-7Ra) and blocks binding between IL-7 IL-7Ra has been produced. Cell surface staining with A7R34 demonstrated is expressed in both B- T-cell lineages. In bone marrow, immature B-lineage cells do not express IgM were IL-7Ra+. thymus, was detected CD4-8- T also CD4 or CD8 single-positive but CD4+8+ double-positive cells. peripheral lymphoid tissues, major cell types IL-7Ra. Addition of to a long-term B-precursor-cell culture inhibited proliferation cells, indicating an absolute requirement for vitro B-cell genesis. Consistent this result, continuous injection into adult mouse resulted decrease B-precursor thymocytes, whereas considerable fraction mature B tissues persisted over 2 weeks experiment. When started from day 14 gestation, it possible produce mice lack These results indicate essential molecule generation murine marrow respectively. Moreover, would be sole system regulating these processes.