Development of a Steady-State Oral Microbial Biofilm Community using the Constant-Depth Film Fermenter

摘要: The complexity of biofilm communities like dental plaque suggests that laboratory model growth systems may help to understand their structure and function. This study describes the use a constant-depth film fermenter (CDFF) investigate formation by nine-membered community oral bacteria. was grown steady state in chemostat incubated anaerobically. output fed into CDFF aerobically. Viable counts for each species from at showed major differences; however, all nine organisms were present under both conditions. There evidence succession during with obligately anaerobic only establishing after several days. A steady-state achieved which remained stable over time. Electron microscopy spatial differentiation what appeared be Neisseria subflava dominant near upper surface Fusobacterium nucleatum largely confined middle portion.