Hepatitis C virus-specific CTL responses in PBMC from chimpanzees with chronic hepatitis C: determination of CTL and CTL precursor frequencies using a recombinant canarypox virus (ALVAC).
作者： Marielle Cohard , Qingyan Liu , Marion Perkus , Ethel Gordon , Betsy Brotman
DOI: 10.1016/S0022-1759(98)00054-4
关键词:
摘要: Abstract The aim of this study was to evaluate HCV-cytotoxic T lymphocyte response from PBMC in bulk CTL assays and precursor analyses using vitro stimulation with canarypox virus (ALVAC) expressing HCV-capsid/E1/E2/NS2/NS3 antigens. Canarypox is naturally host-range restricted does not replicate or cause cytopathology on mammalian cells. were obtained four chimpanzees chronic hepatitis C infection one uninfected chimpanzee. culture frequencies found three the chronically infected ALVAC stimulation. No detected specific for capsid, NS2 NS3 proteins ranged between 1/2663 1/27202. correlation observed percent cytolysis frequencies. This method may prove useful assessing HCV–CTL virological histological status.
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