An enzymatic method for preparation of homopolymannuronate blocks and strictly alternating sequences of mannuronic and guluronic units

摘要: Abstract Two Pseudomonas aeruginosa alginates were lysed by an overexpressed polymannuronate lyase AlxM B (only acting on two or more consecutive, nonacetylated mannuronate units) to prepare either blocks (poly-M blocks) with dp∼30, strictly alternating sequences of mannuronic and guluronic acid (poly-MG dp>20. The poly-M obtained lysis a P. that has 50% O-acetylation at C-2 C-3. poly-MG from alginate contained both guluronate residues. polysaccharide was first deacetylated then treated the excise units alternating-MG blocks. Both types should have potent biological effects provide useful specific substrates for characterisation other lyases.