Variations in DNA methylation patterns during the cell cycle of HeLa cells.

摘要: DNA methylation has been viewed as a stable component of the epigenome, which is established during development and fixed thereafter. We show here using nearest neighbor analysis, immunocytochemistry, high performance capillary electrophoresis that pattern varies in HeLa cells single cell cycle. Immunocytochemical analysis primary human fibroblasts shows similar variations. The global levels decreased G1 increase S phase Since there was little change repetitive sequences throughout cycle, we examined unique CpG island microarray. Hybridization with methylated from cycle revealed 174 CG-containing were differentially between S. 75% all variations detected represented hypomethylation at G0, changes occurring ...