Spatiotemporal analysis of DNA repair using charged particle radiation.

摘要: Approaches to visualise the dynamics of DNA lesion processing substantially contributes understanding hierarchical organisation damage response pathways. Charged particle irradiation has recently emerged as a tool generate discrete sites subnuclear by its means extremely localised dose deposition at low energies, thus facilitating spatiotemporal analysis repair events. In addition, they are high interest for risk estimations human space exploration (e.g. mars mission) in energy regime (HZE). this short review we will give examples application charged study aspects recognition and context recent achievements field. Beamline microscopy allows determining exact kinetics repair-related proteins after with different particles that induce densities. The classification into fast recruited like DNA-PK or XRCC1 slower ones 53BP1 MDC1 helps establish subsequent Additionally, motional lesions induced traversing proved information about mobility DSBs. Increased absence large scale motion direct consequences on formation chromosomal translocations and, thus, mechanisms cancer formation. microbeams offer interesting perspective precise nuclear targeting defined number ions, avoiding Poisson distribution traversals inherent broad beam experiments. With help microbeam, geometrical patterns ions can be applied spatial repair.