The Absence of an Early Calcium Response to Heavy-Ion Radiation in Mammalian Cells

摘要: Intracellular calcium is an important second messenger that regulates many cell functions. Recent studies have shown ions can also regulate the cellular responses to ionizing radiation. However, previous data are restricted cells treated with low-LET radiations (X rays, gamma rays and beta particles). In this work, we investigated levels in exposed heavy-ion radiation of high LET. The experiments were performed at single ion hit facility GSI microprobe. Using a built-in online imaging system, intracellular concentrations examined HeLa human foreskin fibroblast AG1522-D before after irradiation 4.8 MeV/nucleon carbon or argon ions. Although experiment was sensitive enough detect response other known stimuli, no found these two types. We has impact on oscillation induced by hypoxia stress cells.