Ion Irradiation as a Tool to Reveal the Spatiotemporal Dynamics of DNA Damage Response Processes

摘要: In response to genotoxic exposure, mammalian cells have developed efficient DNA repair and cell-cycle checkpoint mechanisms that allow coping with both endogenous exogenous sources of damage. Imaging approaches visualize the dynamics repair-related proteins at locally restricted regions damage substantially contributed understanding biochemical processes involved in these pathways. Taking advantage their extremely localized dose deposition, charged particle beams recently emerged as a tool generate discrete sites subnuclear The application this focal type irradiation study spatiotemporal aspects recognition is presented context recent advancements field.