Vascular endothelial growth factor (VEGF)-C synergizes with basic fibroblast growth factor and VEGF in the induction of angiogenesis in vitro and alters endothelial cell extracellular proteolytic activity

摘要: Vascular endothelial growth factor-C (VEGF-C) is a recently characterized member of the VEGF family angiogenic polypeptides. We demonstrate here that VEGF-C in vitro when added to bovine aortic or lymphatic (BAE and BLE) cells but has little no effect on microvascular (BME) cells. As reported previously for VEGF, basic fibroblast factor (bFGF) induced synergistic response all three lines. Unexpectedly, also synergized assessed BAE Characterization receptor (VEGFR) expression revealed BME, BAE, BLE cell lines express VEGFR-1 -2, whereas assessed, only VEGFR-3. increases plasminogen activator (PA) activity this accompanied by concomitant increase PA inhibitor-1. Addition a2-antiplasmin BME co-treated with bFGF partially inhibited collagen gel invasion. These results demonstrate, first, acting concert potent induction angiogenesis and, second, like bFGF, capable altering extracellular proteolytic activity. observations highlight notion context, i.e., an angiogenesis-regulating cytokine depends presence concentration other cytokines pericellular environment responding cell. J. Cell. Physiol. 177:439‐452, 1998. q 1998 Wiley-Liss, Inc.