Moderate expression of Wnt signaling genes is essential for porcine parthenogenetic embryo development.
作者： Yongye Huang , Hongsheng Ouyang , Wanhua Xie , Xianju Chen , Chaogang Yao
DOI: 10.1016/J.CELLSIG.2013.01.001
关键词:
摘要: Parthenogenetic embryos are invariably lost in mid-gestation, possibly due to the lack of paternal genome and consequent induction aberrant gene expression. Wnt signaling is essential for embryonic development; however, studies this pathway porcine parthenogenetic have been limited. Here, role was studied. In vivo were used as controls. Single cell quantitative real-time PCR showed that down-regulated embryos. Furthermore, immunofluorescence staining demonstrated embryo development largely unaffected by inhibition with IWP-2, but blastocyst hatching trophectoderm blocked. addition, improved activation BIO. However, developmental competency embryos, including hatching, impaired apoptosis induced upon excessive signaling. These findings constitute novel evidence important pre-implantation its down-regulation may lead low rate blastocysts.
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